pMAL-p5x載體基本信息
| 載體名稱: | pMAL-p5x | 
|---|---|
| 質粒類型: | 大腸桿菌表達載體;蛋白表達與純化 | 
| 高拷貝/低拷貝: | 低拷貝 | 
| 啟動子: | tac | 
| 克隆方法: | 多克隆位點,限制性內切酶 | 
| 載體大小: | 5752bp | 
| 5' 測序引物及序列: | -- | 
| 3' 測序引物及序列: | -- | 
| 載體標簽: | MBP tag | 
| 載體抗性: | Ampicillin | 
| 篩選標記: | -- | 
| 備注: | -- | 
| 產品目錄號: | N8109S | 
| 穩定性: | -- | 
| 組成型: | -- | 
| 病毒/非病毒: | -- | 
				

 
			
The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, 
where the protein of interest can be cleaved from MBP with the specific protease Factor Xa.
MBP fusions made with this vector include an N-terminal signal sequence, so the 
fusion protein is directed to the periplasm. The MBP has been engineered for tighter 
binding to amylose resin.
A gene or open reading frame is inserted into a restriction site of the vector polylinker, 
in the same translational reading frame as the malE gene (encoding maltose-binding protein).
The fusion protein thus produced can be purified by amylose affinity chromatography. The 
sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI 
site. This allows the protein of interest to be cleaved from maltose-binding protein with 
the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) 
will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived 
residues on the protein of interest.