Hs 600.T細胞 
	
		數量:	大量
	
	
		生長狀態:	貼壁生長
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		運輸方式:	凍存運輸
	
	
		ATCC Number:	CRL-7360?
	
	
		器官來源:	皮膚
	
	
		相關**:	黑色素瘤
	
	
		Designations:	Hs 600.T
	
	
		Hs 600.T細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:
	
	
		Source:	Organ: skin
	
	
		Hs 600.T細胞Disease: melanoma
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
	
	
		Cytogenetic Analysis:	modal number = 46; range = 44 to 46
	
	
		Comments:	Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
	
	
		Please see the NBL Repository description.
	
	
		Propagation: Hs 600.T細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
	
	
		Medium Renewal: Every 2 to 3 days
	
	
		Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
	
	
		Add fresh culture medium, aspirate and dispense into new culture flasks.
	
	
		Preservation:	culture medium 95%; DMSO, 5%
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
	
	
		recommended serum:Hs 600.T細胞ATCC 30-2020