Hs 696細胞 
	
		年限:	43 years
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		ATCC Number:	HTB-151?
	
	
		相關(guān)**:	腺癌
	
	
		細胞形態(tài):	上皮樣
	
	
		生長狀態(tài):	貼壁生長
	
	
		運輸方式:	凍存運輸
	
	
		數(shù)量:	大量
	
	
		Designations:	Hs 696
	
	
		Depositors:	 RB Owens
	
	
		Hs 696細胞Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Disease: adenocarcinoma
	
	
		Derived from metastatic site: bone (sacrum)
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Tumorigenic:	No
	
	
		Cytogenetic Analysis:	Y chromosome present
	
	
		Isoenzymes:	AK-1, 1
	
	
		ES-D, 1
	
	
		Hs 696細胞G6PD, B
	
	
		GLO-I, 1-2
	
	
		Me-2, 1
	
	
		PGM1, 1
	
	
		PGM3, 1
	
	
		Age:	43 years
	
	
		Gender:	male
	
	
		Ethnicity:	Asian
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Subculturing:	Subcultivation Ratio: Hs 696細胞A subcultivation ratio of 1:2 to 1:4 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
	
	
		Preservation:	Culture medium, 95%; DMSO, 5%
	
	
		References:	22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
	
	
		22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. Hs 696細胞PubMed: 283258