Hs 860.T細胞 
	
		運輸方式:	瓶裝運輸
	
	
		數量:	大量
	
	
		ATCC Number:	CRL-7595?
	
	
		相關**:	骨肉瘤
	
	
		生長狀態:	貼壁生長
	
	
		細胞形態:	成纖維樣
	
	
		年限:	22 years
	
	
		是否是腫瘤細胞:	1
	
	
		物種來源:	人
	
	
		器官來源:	骨
	
	
		Designations:	Hs 860.T
	
	
		Hs 860.T細胞Biosafety Level:	1
	
	
		Shipped:	flask
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	fibroblast
	
	
		
	
	
		Source:	Organ: bone
	
	
		Disease: osteosarcoma
	
	
		Derived from metastatic site: lung
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Cytogenetic Analysis:	modal number = 46; range = 46 to 47
	
	
		Age:	22 years
	
	
		Gender:	male
	
	
		Ethnicity: Hs 860.T細胞Caucasian
	
	
		Comments:	Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
	
	
		Please see the NBL Repository description.
	
	
		Note: This item is distributed only within the 50 United States. It is not available for international distribution.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Medium Renewal: Every 2 to 3 days
	
	
		Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Hs 860.T細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
	
	
		Add fresh culture medium, aspirate and dispense into new culture flasks.
	
	
		Preservation:	culture medium 95%; DMSO, 5%
	
	
		Related Products:	Recommended medium Hs 860.T細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
	
	
		recommended serum:ATCC 30-2020