HuNS1細(xì)胞
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-8644?
相關(guān)**： 其他**
細(xì)胞形態(tài)： **樣
運輸方式： 凍存運輸
細(xì)胞類型： **母細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: HuNS1
Depositors: Medical College of Wisconsin Research Foundation, Inc.
HuNS1細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: myeloma
Cell Type: lymphoblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
HuNS1細(xì)胞CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Gender: male
Comments: HuNS1細(xì)胞This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
The cells are HAT sensitive, do not secrete immunoglobulin and are negative for Epstein-Barr virus.
The line is suitable for use as a fusion partner for human B cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: Complete Culture Medium, 95%; DMSO, 5%.
Storage temperature: liquid nitrogen vapor phase
References: 3864: Winkelhake JL. HuNS1細(xì)胞Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988
