SK-N-FI細胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

細胞類型： 其他細胞類型

是否是腫瘤細胞： 1

物種來源： 人

器官來源： 大腦

ATCC Number： CRL-2142?

相關(guān)**： 神經(jīng)母細胞瘤

運輸方式： 凍存運輸

細胞形態(tài)： 上皮樣

年限： 11 years

Designations: SK-N-FI

SK-N-FI細胞Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow

Cell Type: neuroblast;

Cellular Products: tumor necrosis factor (TNF)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1979

Receptors: SK-N-FI細胞tumor necrosis factor (TNF) type B, expressed

tumor necrosis factor (TNF) type A, expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,14

D16S539: 11,12

D5S818: 12,13

D7S820: 8,9

THO1: 6,9.3

TPOX: 8,9

vWA: 16,17

Age: 11 years

Gender: male

Ethnicity: Caucasian

Comments: SK-N-FI is a neuroblastoma cell line derived in 1979 from a bone marrow metastasis from a 11 year old Caucasian male with poorly differentiated embryonal neuroblastoma.

SK-N-FI細胞The cells exhibit high MDR1 expression.

Recombinant tumor necrosis factor (TNF) stimulates the proliferation of SK-N-FI cells in both serum free medium and fetal bovine serum supplemented medium, but has no effect in medium without insulin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

O.1 mM Non-Essential Amino Acids (NEAA)

fetal bovine serum to a final concentration of 10%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: SK-N-FI細胞A subcultivation ratio of 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

23098: Goillot E, et al. Tumor necrosis factor as an autocrine growth factor for neuroblastoma. Cancer Res. 52: 3194-3200, 1992. PubMed: 1317260