M059J細胞

細胞類型： 其他細胞類型

是否是腫瘤細胞： 1

物種來源： 人

數量： 大量

年限： 33 years

器官來源： 大腦

生長狀態： 貼壁生長

ATCC Number： CRL-2366?

相關**： 其他**

細胞形態： 成纖維樣

運輸方式： 凍存運輸

Designations: M059J

M059J細胞Depositors: J Allalunis-Turner, RS Day

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: brain

Disease: malignant glioblastoma; glioma

Cell Type: glial cell;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 14

D16S539: 10,12

D5S818: 11,12

D7S820: 10,12

M059J細胞THO1: 9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: aneuploid; Y chromosome is present

Age: 33 years

Gender: male

Comments: The cells were isolated concurrently from the same tumor specimen as M059K (see CRL-2365).

M059J cells lack DNA-dependent protein kinase activity, while M059K cells express normal levels of DNA-dependent protein kinase

M059J cells are approximately 30-fold more sensitive to ionizing radiation than M059K cells

M059J cells are more sensitive than M059K cells to the cytotoxic effects of bleomycin, N,N-bis(2-choroethyl)-N-nitrosourea and nitrogen mustard

M059J cells are deficient in repair of DNA double strand breaks

The cells are negative for glial fibrillary acidic protein (GFAP)

Together, M059K and M059J provide a useful model system in which to study the role of DNA protein kinase in cellular and molecular processes involving DNA damage recognition and repair

M059J cells were isolated from a tumor specimen taken from a 33 year old male with untreated malignant glioblastoma

Propagation: M059J細胞ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-2365

References: 33940: Allalunis-Turner MJ, et al. Isolation of two lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs. Radiat. Res. 134: 349-354, 1993. PubMed: 8316628

33942: Lees-Miller SP, et al. M059J細胞Absence of p350 subunit of DNA activated protein kinase from a radiosensitive human cell line. Science 267: 1183-1185, 1995. PubMed: 7855602

38596: Allalunis-Turner J, et al. Intact G2-phase checkpoint in cells of a human cell line lacking DNA-dependent protein kinase activity. Radiat. Res. 147: 284-287, 1997. PubMed: 9052673

38598: Allalunis-Turner MJ, et al. Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line. Radiat. Res. 144: 288-293, 1995. PubMed: 7494872

38599: Wang J, et al. Radiation-induced damage in two human glioma cell lines as measured by the nucleoid assay. Anticancer Res. 17: 4615-4618, 1997. PubMed: 9494578