BeWo細(xì)胞

ATCC Number： CCL-98?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 胎盤

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

Designations: BeWo

Depositors: RA Pattillo

BeWo細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: placenta

Disease: choriocarcinoma

Cellular Products: hormones; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin

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Virus Susceptibility: Human poliovirus 3

Vesicular stomatitis virus

DNA Profile (STR): BeWo細(xì)胞Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 9,11

D16S539: 13,14

D5S818: 10,11

D7S820: 10,12

THO1: 9,9.3

TPOX: 8

vWA: 16

Cytogenetic Analysis: modal number = 86; range = 71 to 178.

Stemline number is hypotetraploid. Karyotype relatively stable within stemline number.

Isoenzymes: G6PD, B

Gender: male

Comments: The cells are positive for keratin by immunoperoxidase staining.

Propagation:BeWo細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: 3 to 4 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 1219: Pattillo RA, et al. BeWo細(xì)胞Human hormone production in vitro. Science 159: 1467-1469, 1968. PubMed: 5753554

22139: Pattillo RA, et al. Control mechanisms for gonadotrophic hormone production in vitro. In Vitro 6: 205-214, 1970. PubMed: 5535575

22343: Pattillo RA, et al. Estrogen production by trophoblastic tumors in tissue culture. J. Clin. Endocrinol. Metab. 34: 59-61, 1972. PubMed: 4332667

26271: Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 28: 1231-1236, 1968. PubMed: 4299001

26272: Hertz R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc. Soc. Exp. Biol. Med. 102: 77-81, 1959. PubMed: 14401422

26273: Pattillo RA, et al. The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann. N.Y. Acad. Sci. 172: 288-298, 1971. PubMed: 5289994

32279: Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797

32858: Heckert LL, et al. The cAMP response elements of the alpha subunit gene bind similar proteins in trophoblasts and gonadotropes but have distinct functional sequence requirements. J. Biol. Chem. 49: 31650-31656, 1996. PubMed: 8940185