HT-1197細(xì)胞

年限： 44 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1473?

相關(guān)**： 腫瘤

器官來源： 膀胱

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: HT-1197

Depositors: S Rasheed

HT-1197細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: urinary bladder

Disease: carcinoma

Cellular Products: fibrinolytic activity

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Tumorigenic: Yes

Isoenzymes: G6PD, B

Age: 44 years

Gender: HT-1197細(xì)胞male

Ethnicity: Caucasian

Comments: The cells will also grow in soft agar.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. HT-1197細(xì)胞Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. Incubate cultures at 37?C.

Subcultivation Ratio: Subcultivation Ratio: 1:5 to 1:10

Medium Renewal: HT-1197細(xì)胞Twice per week

Preservation: Storage temperature: liquid nitrogen vapor phase

Related Products: purified DNA:ATCC CRL-1473D

References: 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628