A-253細(xì)胞
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
ATCC Number： HTB-41?
運(yùn)輸方式： 凍存運(yùn)輸
相關(guān)**： 上皮細(xì)胞癌
年限： 54 years *****
器官來源： 其他
是否是腫瘤細(xì)胞： 1
物種來源： 人
Designations: A-253
A-253細(xì)胞Depositors: G Todaro
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: submaxillary salivary gland
Disease: epidermoid carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12,13
D13S317: 9,12
D16S539: 12
D5S818: 11
D7S820: 10,12
A-253細(xì)胞THO1: 6,8
TPOX: 8,12
vWA: 16
Cytogenetic Analysis: near triploid with at least 6 markers
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Age: 54 years *****
Gender: male
Ethnicity: Caucasian
Comments: The A253 line is one of thirteen to be isolated and partially characterized through trial studies on a series of 200 human tumors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: A-253細(xì)胞Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove the trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
23218: Giard DJ, et al. In vitro cultivation of human tumors: A-253細(xì)胞establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
