NCI-H292細胞

年限： 32 years

ATCC Number： CRL-1848?

數量： 大量

相關**： 其他**

運輸方式： 凍存運輸

器官來源： 肺

細胞形態： 上皮樣

生長狀態： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

Designations: NCI-H292 [H292]

Depositors: AF Gazdar

NCI-H292細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: mucoepidermoid pulmonary carcinoma

Cellular Products: keratin; vimentin

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Applications: The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein.

The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase.

This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma.

Virus Susceptibility: Hepatitis B virus

NCI-H292細胞Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11,12

D16S539: 9,13

D5S818: 13

D7S820: 10

THO1: 8

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: This is a human cell line with near-diploid chromosome counts. The modal chromosome number was 47, occurring in 36% of cells. The rate of cells with a higher ploidy count was 3.9%. Twelve markers were common to most cells. Among them were del(1) (q32.1), der (5)t(5;13) (p15.33;q11), i(5p), der(1)t(1;?) (p34.3;?) and der (6)t(6;7) (p25.3;q21.2). All markers were present in single copy per cell. Normal N1 and N6 were absent. There were two normal X chromosomes. No other abnormalities were detected.

Age: 32 years

Gender: female

Ethnicity: NCI-H292細胞Black

Comments: This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma.

The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum.

The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation.

The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase.

The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer.

The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: NCI-H292細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

22946: Yoakum GH, et al. High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science 222: 385-389, 1983. PubMed: 6194563

23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257