SCC-9細胞

數量： 大量

年限： 25 years

是否是腫瘤細胞： 1

物種來源： 人

生長狀態： 貼壁生長

ATCC Number： CRL-1629?

相關**： 鱗狀細胞癌

運輸方式： 凍存運輸

器官來源： 舌頭

Designations: SCC-9

Depositors: JG Rheinwald

SCC-9細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: tongue

Disease: squamous cell carcinoma

Cellular Products: epidermal keratins; low levels of involucrin

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Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 9

SCC-9細胞D16S539: 10,11

D5S818: 12

D7S820: 8

THO1: 8,9

TPOX: 9,11

vWA: 17

Age: 25 years

Gender: male

Comments: Growth of SCC-9 is enhanced by using a feeder layer of X-irradiated STO cells (ATCC 56-X).

The cells do not grow well in semi-solid medium.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: SCC-9細胞Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. SCC-9細胞PubMed: 7214336