CFPAC-1細胞

年限： 26 years

細胞形態： 上皮樣

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CRL-1918?

相關**： 其他**

生長狀態： 貼壁生長

數量： 大量

器官來源： 胰腺

運輸方式： 凍存運輸

Designations: CFPAC-1

Depositors: RA Schoumacher

CFPAC-1細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: pancreas

Disease: ductal adenocarcinoma; cystic fibrosis

Derived from metastatic site: liver metastasis

Cellular Products: carcinoembryonic antigen (CEA), 9 ng/ml; pancreatic oncofetal antigen (POA), 28 ng/ml; adenocarcinoma associated antigen (ACAA), 5000 ng/ml; CA 19-9 antigen, 12000 units/ml; epithelial keratins

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Applications: CFPAC-1細胞transfection host

Tumorigenic: Yes

Antigen Expression: CA19-9 antigen, 12000 units/ml

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 12

D16S539: 9,11

D5S818: 10,11

D7S820: 8,10

THO1: 8

TPOX: 8

vWA: 17

Cytogenetic Analysis: hyperdiploid; modal number = 73; range = 65 to 75; several translocations were evident; two to three apparently normal copies of number 7 (to which the cystic fibrosis gene has been mapped) were present in every metaphase examined

Age: 26 years

Gender: male

Ethnicity: Caucasian

Comments: This line was derived from a ductal adenocarcinoma (liver metastasis) from a patient with cystic fibrosis.

The cells exhibit ion transport activities consistent with cystic fibrosis and express the product of the CF gene (cystic fibrosis transmembrane regulator, CFTR).

CFPAC-1細胞CFPAC-1 cells show no effect of cAMP agonists, adenyl cyclase stimulators or phosphodiesterase inhibitors on Cl- flux, but do respond to Ca++ ionophores with increase Cl- efflux.

The cells have the most common form of the CF mutation, deletion of three nucleotides resulting in the absence of phenylalanine at position 508.

CFPAC-1 cells have epithelial morphology and polarization with apical microvilli, tight junctions and gap junctions.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: CFPAC-1細胞A subcultivation ratio of 1:3 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 32 hrs

References: 1785: Schoumacher RA, et al. A cystic fibrosis pancreatic adenocarcinoma cell line. Proc. Natl. Acad. Sci. USA 87: 4012-4016, 1990. PubMed: 1692630

22227: McIntosh JC, et al. Pancreatic adenocarcinoma in a patient with cystic fibrosis. Am. J. Med. 85: 592, 1988. PubMed: 3177424