CA-HPV-10細胞

細胞類型： 其他細胞類型

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

細胞形態(tài)： 上皮樣

ATCC Number： CRL-2220?

相關(guān)**： 腺癌

器官來源： 前列腺

生長狀態(tài)： 貼壁生長

年限： 63 years

CA-HPV-10細胞數(shù)量： 大量

Designations: CA-HPV-10

Depositors: DM Peehl

Biosafety Level: 2 [Cells contain human papilloma viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: prostate

Disease: adenocarcinoma

Cell Type: CA-HPV-10細胞human papillomavirus 18 (HPV-18) transfected

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Applications: CA-HPV-10 was derived from cells from a prostatic adenocarcinoma of Gleason Grade 4/4. The cells were transformed by transfection with HPV18 DNA. Incorporation of HPV18 DNA was confirmed by polymerase chain reaction.

Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.

Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted.

Tumorigenic: No

Antigen Expression: kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens

Cytogenetic Analysis: aneuploid; mean chromosome number at passage 26 was 72; 10% of the cells retain the double minutes seen in the source tumor

Age: 63 years

Gender: male

Ethnicity: Caucasian

Comments:CA-HPV-10細胞 CA-HPV-10 was derived from cells from a prostatic adenocarcinoma of Gleason Grade 4/4. The cells were transformed by transfection with HPV18 DNA. Incorporation of HPV18 DNA was confirmed by polymerase chain reaction. Specific amplification of a 160-base pair fragment of the HPV18 E6 transforming region was noted. Immunocytochemical analysis showed expression of keratins 5 and 8 and also the early region 6 (E6) oncoprotein of HPV.

Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:

0.05 mg/ml BPE - provided with the K-SFM kit

5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.

Temperature: 37.0°C

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution; let the culture set incubate at 37C for two minutes. Neutralize the trypsin with 0.1% soybean trypsin inhibitor, and gently dislodge the cells by agitating or tapping the flask. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: CA-HPV-10細胞Every 2 to 3 days

Preservation: Freeze medium: Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 23116: Weijerman PC, et al. Lipofection-mediated immortalization of human prostatic epithelial cells of normal and malignant origin using human papillomavirus type 18 DNA. Cancer Res. 54: 5579-5583, 1994. PubMed: 7923200