NCCIT細胞

生長狀態： 貼壁生長

運輸方式： 凍存運輸

ATCC Number： CRL-2073?

相關**： 其他**

是否是腫瘤細胞： 1

物種來源： 人

數量： 大量

年限： *****

細胞形態： 上皮樣

NCCIT細胞Designations: NCCIT

Depositors: I Damjanov

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Disease: pluripotent embryonal carcinoma; teratocarcinoma

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Tumorigenic: NCCIT細胞Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 11

D16S539: 9,12

D5S818: 10,13

D7S820: 10

THO1: 7,9

TPOX: 8

vWA: 14,18

Age: *****

Gender: male

Ethnicity: Japanese

Comments: NCCIT細胞NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor.

This pluripotent stem cell line is capable of somatic and extraembryonic differentiation.

The undifferentiated cells are equivalent to a stage intermediate between seminoma and embryonal carcinoma.

They will differentiate in response to retinoic acid.

NCCIT cells are negative for keratin.

They are positive for vimentin and placental alkaline phosphatase.

The line was contaminated with mycoplasma, but was cured by the depositor prior to deposit at the ATCC .

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Add fresh medium at the time of subculture

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. NCCIT細胞Add fresh medium, aspirate and dispense into new flasks. Subculture two times weekly.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22742: Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544

22743: Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083