1H3細(xì)胞

ATCC Number： HB-284?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

**類型： IgG2a

1H3細(xì)胞Designations: 1H3

Depositors: Mo Dailey, JW Shepherd

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: 1H3細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human CD62L

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Comments: Animals were immunized with human peripheral blood leukocytes.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.

1H3細(xì)胞The antibody can be used in both immunofluorescence and immunoprecipitation assys.

The cells can be adapted to serum free growth in HB101 medium.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: 1H3細(xì)胞Culture medium, 95%; DMSO, 5%

References: 22158: . . FASEB J. 6: 1142, 1992.