1H11細(xì)胞

ATCC Number： HB-10611?

生長狀態(tài)： 懸浮生長

細(xì)胞形態(tài)： **樣

**類型： IgG1

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

1H11細(xì)胞物種來源： 小鼠

Designations: 1H11

Depositors: Washington Research Foundation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: 1H11細(xì)胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.

Spleen cells were fused with mouse myeloma cells.

The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.

Propagation: 1H11細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22055: Eyre DR. 1H11細(xì)胞Hybridoma cell line producing an antibody to type-I collagen amino-terminal telopeptide. US Patent 5,300,434 dated Apr 5 1994