Le Ana細胞 
	
		生長狀態:	貼壁生長
	
	
		ATCC Number:	CRL-1192?
	
	
		相關**:	馬凡氏綜合征
	
	
		數量:	大量
	
	
		器官來源:	皮膚
	
	
		年限:	2 years
	
	
		運輸方式:	凍存運輸
	
	
		細胞形態:	成纖維樣
	
	
		是否是腫瘤細胞:	0
	
	
		Le Ana細胞物種來源:	人
	
	
		Designations:	Le Ana
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	fibroblast
	
	
		
	
	
		Source:	Organ: skin
	
	
		Disease: Le Ana細胞Marfan syndrome
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Age:	2 years
	
	
		Gender:	female
	
	
		Comments:	The tissue donor suffered from an atypical form of Marfan syndrome.
	
	
		The patient presented with no eye findings, normal height and severe joint laxity.
	
	
		Propagation: Le Ana細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Le Ana細胞Subculture every 7 to 9 days.