TD.1細胞
細胞形態： **樣
**類型： IgG1
生長狀態： 懸浮生長
運輸方式： 凍存運輸
數量： 大量
ATCC Number： CRL-2232?
是否是腫瘤細胞： 0
物種來源： 小鼠
TD.1細胞Designations: TD.1
Depositors: FM Brodsky
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: TD.1細胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human clathrin heavy chain
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Comments: Animals were immunized with the globular N-terminal domain of clathrin heavy chain.
TD.1細胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is useful for blotting of clathrin heavy chains from a variety of species. It does not react with native clathrin in immunofluorescence or immunoprecipitation assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22634: Nathke IS, et al. TD.1細胞Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490
