TE15細胞 
	
		細胞形態(tài):	**樣
	
	
		**類型:	IgM
	
	
		數(shù)量:	大量
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	小鼠
	
	
		生長狀態(tài):	懸浮生長
	
	
		ATCC Number:	HB-206?
	
	
		運輸方式:	凍存運輸
	
	
		Designations:	TE15
	
	
		TE15細胞Depositors:	 BF Haynes
	
	
		Isotype:	IgM
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	suspension
	
	
		Organism:	Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
	
	
		Morphology:	lymphoblast
	
	
		
	
	
		Source:	Cell Type: hybridoma: B lymphocyte;
	
	
		Cellular Products:	immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
	
	
		Permits/Forms: TE15細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Comments:	Animals were immunized with human thymic stroma.
	
	
		Spleen cells were fused with NS-1 myeloma cells.
	
	
		The antibody reacts with granular foci among the cellular swirls of Hassall's bodies.
	
	
		It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
	
	
		TE15細胞The antibody binds specifically to granular foci among the cellular swirls of most Hassall's bodies and to stratum corneum of skin epidermis and foreskin.
	
	
		Tested and found negative for ectromelia virus (mousepox).
	
	
		Propagation:	ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
	
	
		Subculturing:	Medium Renewal: Every 2 to 3 days
	
	
		Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
	
	
		References:	1637: Lobach DF, et al. TE15細胞The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044