G2b.2細(xì)胞
ATCC Number： HB-109?
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： IgG2b
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
G2b.2細(xì)胞Designations: G2b.2
Depositors: P Parham
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: G2b.2細(xì)胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR)
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Comments: Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line.
Spleen cells were fused with NS-1 myeloma cells.
G2b.2細(xì)胞This line is a switch variant of Genox 3.53 (ATCC HB-103).
An IgG2a switch variant of Genox 3.53 also exists (G2a.5, ATCC HB-110).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 994: Parham P, et al. G2b.2細(xì)胞Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068
