Lp1 MAB 2細胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態(tài)： **樣
**類型： IgG2b
ATCC Number： CRL-1770?
運輸方式： 凍存運輸
數(shù)量： 大量
Designations: Lp1 MAB 2
Lp1 MAB 2細胞Depositors: RM McKinney
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1
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Comments: Animals were immunized with the bacterial antigenic subgroup Knoxville 1 (ATCC 33153).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a surface antigen on L. pneumophila and is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lp1 MAB 2細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1190: Joly JR, et al. Development of a standard subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23: 768-771, 1986. PubMed: 3517064
58069: McKinney RM, et al. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Lp1 MAB 2細胞Orig. A255: 91-95, 1983.
