gamma3-11.1細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

ATCC Number： HB-8700?

細(xì)胞形態(tài)： **樣

**類型： IgG1

gamma3-11.1細(xì)胞Designations: gamma3-11.1

Depositors: Takeda Chem. Ind., Ltd.

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: gamma3-11.1細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human gamma interferon

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Tumorigenic: Yes

gamma3-11.1細(xì)胞Comments: Animals were immunized with a synthetic peptide (NH2-Lys-Arg-Lys-Arg- Ser-Gln-Met-Leu-Phe-Arg-Gly-Arg-Arg-Ala-Ser-Gln-COOH) conjugated to thyroglobulin.

Spleen cells were fused with P3X63Ag8.U1 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3909: Ichimori Y, et al. gamma3-11.1細(xì)胞Novel peptide and use thereof. US Patent 4,681,848 dated Jul 21 1987