GL1細胞

數量： 大量

生長狀態： 懸浮生長

運輸方式： 凍存運輸

ATCC Number： HB-253?

細胞形態： **樣

是否是腫瘤細胞： 0

物種來源： 大鼠

**類型： IgG2a

Designations: GL1

Depositors: G Lazlo, KS Hathcock

GL1細胞Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse B7-2 antigen

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Comments: GL1細胞Animals were immunized with LPS activated DBA/2J mouse B cells.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.

The B7-2 antigen is a ligand for CTLA-4 (T cell activation molecule).

B7-2 shows minimal expression on unstimulated B cells, but is expressed at high density on activated B cells.

It is distinct from B7-1 (previously referred to as B7).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

GL1細胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22963: Hathcock KS, et al. Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science 262: 905-907, 1993. PubMed: 7694361

22964: Freeman GJ, et al. Uncovering of functional alternative CTLA-4 counter-receptor in B7- deficient mice. Science 262: 907-909, 1993. PubMed: 7694362

32831: Hancock WW, et al. Costimulatory function and expression of CD40 ligand, CD80, and CD86 in vascularized murine cardiac allograft rejection. Proc. Natl. Acad. Sci. USA 93: 13967-13972, 1996. PubMed: 8943044

41516: Freeman GJ, et al. GL1細胞Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262: 909-911, 1993. PubMed: 7694363