2PK-3細胞

細胞形態： **樣

**類型： IgG2 kappa , IgG2; kappa light chain

細胞類型： B**細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

品系： BALB/c

運輸方式： 凍存運輸

數量： 大量

生長狀態： 懸浮生長

ATCC Number： TIB-203?

相關**： **瘤

2PK-3細胞Designations: 2PK-3

Depositors: NL Warner

Isotype: IgG2 kappa , IgG2; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: BALB/c

Cell Type: B lymphocyte;

Cellular Products: surface immunoglobulin (sIg +)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 2PK-3細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: I-A d; I-E d

Comments: Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.

It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

2PK-3細胞Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 21886: . Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.

22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906

23536: Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413

32236: Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620

58054: Gutman GA, et al. 2PK-3細胞Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803